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Picks primers for PCR reactions

Primer3 picks primers for PCR reactions, considering as criteria:
- oligonucleotide melting temperature, size, GC content, and primer-dimer possibilities,
- PCR product size,
- positional constraints within the source (template) sequence, and
- possibilities for ectopic priming (amplifying the wrong sequence)
- many other constraints.
All of these criteria are user-specifiable as constraints, and some are specifiable as terms in an objective function that characterizes an optimal primer pair.

Source Files

Filename Size Changed Actions
primer3-2.3.7.tar.gz 1.58 MB Download File
primer3.spec 2.79 KB Download File

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