DASS-GUI is a stand-alone program written in C++ that calculates all significant closed sets of a given dataset containing the host sets. Some of the used algorithms are taken from Hollunder et al. (2007). DASS-GUI also allows additional analyses of the identified closed sets: filtering, handling of synonymous names, enrichment analyses, clustering, calculation of means and standard deviations of different numerical features, extraction of the underlying closed set hierarchy and corresponding export as GML, NET, LIBSEA, or EDGE file, as well as comparison (validation) with pre-defined sets.